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ATG9B Antibody (CT)

Rabbit Polyclonal Antibody

  • ICC - ATG9B Antibody (CT) ABV10806
    Immunocytochemistry of ATG9B in HeLa cells with ATG9B antibody at 10 μg/mL
  • WB - ATG9B Antibody (CT) ABV10806
    Western blot analysis of ATG9B in HeLa cell lysate with ATG9B antibody at (A) 1 and (B) 2 μg/mL.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q674R7
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG1
Calculated MW 101019 Da
Additional Information
Gene ID 285973
Positive Control Western Blot: HeLa cell lysate
Immunocytochemistry: HeLa cells
Immunoflorescence: HeLa cells
Application & Usage Western Blot: 1 - 2 µg/ml, Immunocytochemistry: 10 µg/ml, Immunoflorescence : 20 µg/ml, ELISA. However, the optimal conditions should be determined individually.
Other Names Autophagy-related protein 9B, APG9L2, APG9-like 2, Nitric oxide synthase 3-overlapping antisense gene protein, NOS3AS
Target/Specificity ATG9B
Antibody Form Liquid
Appearance Colorless liquid
Formulation 100 µg (1 mg/ml) in 1X PBS containing 0.02% sodium azide.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsATG9B Antibody (CT) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATG9B
Synonyms APG9L2, NOS3AS
Function Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle (By similarity).
Cellular Location Cytoplasmic vesicle, autophagosome membrane; Multi-pass membrane protein. Note=Under amino acid starvation or rapamycin treatment, redistributes from a juxtanuclear clustered pool to a dispersed peripheral cytosolic pool. The starvation- induced redistribution depends on ULK1 and ATG13
Tissue Location Highly expressed in placenta (trophoblast cells) and pituitary gland. Not expressed in vascular endothelial
Research Areas
Citations (0)

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Autophagy, the process of bulk degradation of cellular proteins thro µgh an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) thro µgh phosphorylation of autophagy protein APG1. ATG9B plays a role in autophagy and it's highly expressed in placenta and pituitary gland.

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$ 325.00
Cat# ABV10806
(40 western blots)
Availability: 5-7days
Bulk Size
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