|Application ||WB, IF, ICC, E|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||101019 Da|
|Positive Control||Western Blot: HeLa cell lysate|
Immunocytochemistry: HeLa cells
Immunoflorescence: HeLa cells
|Application & Usage||Western Blot: 1 - 2 µg/ml, Immunocytochemistry: 10 µg/ml, Immunoflorescence : 20 µg/ml, ELISA. However, the optimal conditions should be determined individually.|
|Other Names||Autophagy-related protein 9B, APG9L2, APG9-like 2, Nitric oxide synthase 3-overlapping antisense gene protein, NOS3AS|
|Formulation||100 µg (1 mg/ml) in 1X PBS containing 0.02% sodium azide.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||ATG9B Antibody (CT) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle (By similarity).|
|Cellular Location||Cytoplasmic vesicle, autophagosome membrane; Multi-pass membrane protein. Note=Under amino acid starvation or rapamycin treatment, redistributes from a juxtanuclear clustered pool to a dispersed peripheral cytosolic pool. The starvation- induced redistribution depends on ULK1 and ATG13|
|Tissue Location||Highly expressed in placenta (trophoblast cells) and pituitary gland. Not expressed in vascular endothelial|
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Provided below are standard protocols that you may find useful for product applications.
Autophagy, the process of bulk degradation of cellular proteins thro µgh an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) thro µgh phosphorylation of autophagy protein APG1. ATG9B plays a role in autophagy and it's highly expressed in placenta and pituitary gland.
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