|Application ||WB, IHC, E|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||26253 Da|
|Positive Control||Western Blot: K562 cell lysate|
Immunohistochemistry: Human liver tissue
|Application & Usage||Western Blot: 0.5-2 µg/ml, Immunohistochemistry: 2.5 µg/ml, ELISA. However, the optimal conditions should be determined individually.|
|Other Names||Damage-regulated autophagy modulator|
|Formulation||100 µg (1 mg/ml) in 1X PBS containing 0.02% sodium azide.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||DRAM Antibody (NT) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Lysosomal modulator of autophagy that plays a central role in p53/TP53-mediated apoptosis. Not involved in p73/TP73- mediated autophagy.|
|Cellular Location||Lysosome membrane; Multi-pass membrane protein|
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Damage-regulated autophagy modulator (DRAM) is a p53 target gene encoding a lysosomal protein that induces autophagy, a process that degrades cytosolic proteins and organelles. It has been s µggested that activation of DRAM by p53 is simultaneous to the activation by p53 of one or more proapoptotic genes such as PUMA, Bax, etc., and that the signaling pathways regulated by these genes promote a full cell death response. By itself, DRAM cannot induce apoptosis, but the fact that it is inactivated in certain cancers highlights the importance of DRAM and s µggests that autophagy may play a more important role in cancer than initially suspected. At least two different isoforms of DRAM are known to exist.
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