|Application ||WB, IHC, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||42203 Da|
|Application & Usage||Western blot analysis (0.5-4 µg/ml). Per researcher’s feedback, it can also be used in immunoprecipitation (4-8 µg/ml), and Immunohistochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually.|
|Other Names||Fractalkine; C-X3-C motif chemokine 1; Neurotactin; CX3C membrane-anchored chemokine; Small-inducible cytokine D1|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-Fractalkine polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Fractalkine Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||FKN, NTT, SCYD1|
|Function||The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1.|
|Cellular Location||Cell membrane; Single-pass type I membrane protein|
|Tissue Location||Small intestine, colon, testis, prostate, heart, brain, lung, skeletal muscle, kidney and pancreas|
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Provided below are standard protocols that you may find useful for product applications.
Fractalkine, also known as neurotactin, is a membrane-bound CX3C Chemokine. The mature protein is part of a 397-amino acid precursor and consists of a chemokine domain of 76 amino acids, a mucin stalk of 241 amino acids, a putative transmembrane domain of 18 amino acids, and an intracellular tail of 37 amino acids. Within the chemokine domain, the first two cysteine residues are separated by 3 amino acids (CX3C). Fractalkine message is found in high abundance in the brain, kidney, lung and heart tissues. Fractalkine is chemotactic for monocytes and other leukocytes including NK cells and may play a role in brain inflammation.
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