|Application ||WB, E|
|Calculated MW||16091 Da|
|Positive Control||ELISA: Recombinant mGM-CSF|
|Application & Usage||1) WB: Use 0.1-0.2 µg/ml. The detection limit for recombinant mGM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
2) ELISA: Use 0.5 - 2.0 µg/ml (100 µl/well antibody solution)
3) Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mGM-CSF (0.60 ng/ml), a concentration of 0.012-0.020 µg/ml of this antibody is required
|Other Names||CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, MGC131935|
|Formulation||A sterile filtered antibody solution in PBS, pH 7.2.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||GM-CSF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.|
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Provided below are standard protocols that you may find useful for product applications.
GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity.
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