|Application ||WB, E|
|Calculated MW||15909 Da|
|Positive Control||ELISA: Recombinant hIL-9|
|Application & Usage||1) WB: Use 0.1-0.2 µg/ml. The detection limit for recombinant human IL-9 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.|
2) ELISA: Use 0.5 - 2.0 µg/ml (100 µl/well antibody solution)
3) Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-9 (5.0 ng/ml), a concentration of 1.0-2.0 µg/ml of this antibody is required.
|Other Names||p40 cytokine, T-cell growth factor p40|
|Formulation||A sterile filtered antibody solution in PBS, pH 7.2.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||IL-9 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Supports IL-2 independent and IL-4 independent growth of helper T-cells.|
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Provided below are standard protocols that you may find useful for product applications.
IL-9 is an immunoregulatory cytokine produced by IL-2 activated Th2 lymphocytes. IL-9 enhances the proliferation of T lymphocytes, mast cells, erthroid precursor cells and megakaryoblastic leukemia cell lines. Over-expression of IL-9 has been implicated in the pathogenesis of anaplastic lymphoma and Hodgkin's disease. Whereas murine IL-9 can function on human cells, human IL-9 is inactive on mouse cells.
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