|Application ||WB, IHC, E|
|Calculated MW||17504 Da|
|Positive Control||ELISA: Recombinant hIL-17A|
IHC: Human breast invasive ductal carcinoma tissue section
|Application & Usage||1) WB: Use 0.1-0.2 µg/ml. The detection limit for recombinant human IL-17A is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.|
2) ELISA: Use 0.5 - 2.0 µg/ml (100 µl/well antibody solution)
3) Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-17A (50.0 ng/ml), a concentration of 0.9-1.3 µg/ml of this antibody is required.
4) Immunohistochemistry: 0.25 µg/ml with an overnight incubation at 4°C.
|Other Names||CTLA-8, IL-17, IL-17A, Cytotoxic T-lymphocyte associated antigen 8|
|Formulation||A sterile filtered antibody solution in PBS, pH 7.2.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||IL-17A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Ligand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (PubMed:18684971). Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines (PubMed:8676080).|
|Tissue Location||Restricted to activated memory T-cells.|
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Provided below are standard protocols that you may find useful for product applications.
The originally described IL-17 protein, now known as IL-17A, is a homodimer of two 136 amino acid chains, secreted by activated T-cells that act on stromal cells to induce production of proinflammatory and hematopoietic bioactive molecules. Today, IL-17 represents a family of structurally-related cytokines that share a highly conserved C-terminal region but differ from one another in their N-terminal regions and in their distinct biological roles. The six known members of this family, IL-17A through IL-17F, are secreted as homodimers. IL-17A exhibits cross-species bioactivity between human and murine cells.
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