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IL-1 beta Antibody

Rabbit Polyclonal Antibody

     
  • WB - IL-1 beta Antibody ABV10845
    Western blot analysis with recombinant human IL-1. Lane 1: 10 ng; Lane 2: 50 ng; Lane 3: 250 ng; Lane 4: 1μg.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P01584
Other Accession EAW73607
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 30748 Da
Additional Information
Gene ID 3553
Application & Usage Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Recombinant human IL-1β can be used as a positive control.
Other Names IL-1 beta, IL1 beta, IL 1 beta, Interluekin-1 beta, Interluekin 1 beta, interluekin
Target/Specificity IL-1b
Antibody Form Liquid
Appearance Colorless liquid
Formulation 100 µg (0.5 mg/ml) affinity purified rabbit anti-human IL-1β polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsIL-1 beta Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IL1B
Synonyms IL1F2
Function Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells.
Cellular Location Cytoplasm, cytosol. Lysosome. Secreted, exosome {ECO:0000250|UniProtKB:P10749}. Cytoplasmic vesicle, autophagosome. Secreted Note=The precursor is cytosolic. In response to inflammasome- activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B- containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive. {ECO:0000250|UniProtKB:P10749, ECO:0000269|PubMed:11728343, ECO:0000269|PubMed:15192144, ECO:0000305|PubMed:24201029}
Tissue Location Expressed in activated monocytes/macrophages (at protein level).
Research Areas
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Background

IL-1β is a potent immuno-modulator that mediates a wide range of immune and inflammatory responses including the activation of B and T cells. Human IL-1β is a 17.0 kDa protein containing 153 amino acid residues.

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$ 89.00
$ 245.00
Cat# ABV10845
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