|Application ||WB, E|
|Calculated MW||20187 Da|
|Positive Control||Recombinant rat IL-7|
|Application & Usage||1) WB: Use 0.1-0.2 µg/ml. The detection limit for recombinant human IL-7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.|
2) ELISA: Use 0.5 - 2.0 µg/ml (100 µl/well antibody solution)
3) Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-7 (1.0 ng/ml), a concentration of 0.04 - 0.06 µg/ml of this antibody is required.
|Other Names||IL-7, IL7, IL 7, Interluekin-7, Interluekin 7, interluekin|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-rat IL-7 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5mM EDTA and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||IL-7 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation.|
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Provided below are standard protocols that you may find useful for product applications.
IL-7 is a hematopoietic growth factor which affects primarily early B and T cells. Produced by thymic stromal cells, spleen cells and keratinocytes, IL-7 can also co-stimulate the proliferation of mature T cells in combination with other factors such as Con A and IL-2. Human and murine IL-7 is cross-species reactive.
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