|Application ||WB, E|
|Application & Usage||Western blot analysis (0.5-1 µg/ml) and ELISA (2-4 µg/ml). However, the optimal conditions should be determined individually.|
|Other Names||IL-17, IL17, IL 17, Interluekin-17, Interluekin 17, interluekin|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-human IL-17 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 10 mM EDTA, and 0.02% thymersal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||IL-17 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
IL-17 was identified from a CD4+ T cells DNA library. IL-17 can be induced from primary peripheral blood CD4+ T cells upon stimulation. The cytokine exhibits a high degree of amino acid identity with HVS13, an open reading frame from a T lymphotropic Herpesvirus saimiri and with murine CTLA8. Supernatant from cells transfected with IL-17 induced IL-16 and IL-18 production and enhanced the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. IL-17 is a disulfide-linked homodimer of two 31.0 kDa subunits, each containing 136 amino acid residues.
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