|Application ||WB, IHC, E|
|Calculated MW||184667 Da|
|Positive Control||Western Blot:3T3 cell Lysate|
IHC: Human brain tissue
|Application & Usage||Western Blot: 1 µg/ml, IHC: 2.5 µg/ml, ELISA. However, the optimal conditions should be determined individually.|
|Other Names||SH3 and multiple ankyrin repeat domains 3, ProSAP2, PSAP2, SPANK-2, Proline-rich synapse-associated protein 2|
|Formulation||100 µg (1 mg/ml) in 1X PBS containing 0.02 % sodium azide.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||SHANK3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||KIAA1650, PROSAP2, PSAP2|
|Function||Major scaffold postsynaptic density protein which interacts with multiple proteins and complexes to orchestrate the dendritic spine and synapse formation, maturation and maintenance. Interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and HOMER, respectively, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction through the interaction with Arp2/3 and WAVE1 complex as well as the promotion of the F-actin clusters. By way of this control of actin dynamics, participates in the regulation of developing neurons growth cone motility and the NMDA receptor-signaling. Also modulates GRIA1 exocytosis and GRM5/MGLUR5 expression and signaling to control the AMPA and metabotropic glutamate receptor- mediated synaptic transmission and plasticity. May be required at an early stage of synapse formation and be inhibited by IGF1 to promote synapse maturation.|
|Cellular Location||Cytoplasm. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, dendritic spine. Note=In neuronal cells, extends into the region subjacent to the postsynaptic density (PSD).|
|Tissue Location||Expressed in the cerebral cortex and the cerebellum|
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SH3 and multiple ankyrin repeat domains 3 (SHANK3), a member of the Shank gene family, plays a role in synapse formation and dendritic spine maturation. Shank proteins (Shank 1-3) containing PDZ domains are scaffold proteins of the postsynaptic density (PSD) that connect neurotransmitter receptors and ion channels proteins to the actin cytoskeleton and G-protein-coupled signaling pathways. Transcript splice variation in the Shank family influences the spectrum of Shank-interacting proteins in the PSDs of adult and developing brain to ensure normal development. Mutations of SHANK3 are a cause of autism spectrum disorder (ASD) and the neurological symptoms of 22q13.3 deletion syndrome.
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