|Application & Usage||Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. RAW264.7 cells treated with LPS (15 hr) can be used as a positive control.|
|Other Names||Chemokine, CC motif, ligand 5, CCL5, CCL-5, CCL 5|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-RANTES polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||RANTES Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
RANTES (regulated upon activation, normal T-cell expressed and secreted) is a 70-amino acid CC chemokine originally cloned from T-cell-specific sequences-enriched cDNA libraries. Both human and murine RANTES are chemotactic for monocytes. Human RANTES is also a chemoattractant for T-cells and eosinophils, as well as an activator of basophils for histamine release. In contrast to other CC chemokines, RANTES is only slowly expressed after activation of leukocytes. It is involved in a number of pathological conditions like chronic inflammation and autoimmune diseases.
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