|Application ||WB, ICC, E|
|Calculated MW||147866 Da|
|Positive Control||Western Blot: HeLa cell Lysate|
IHC: HeLa cells
|Application & Usage||Western Blot: 1 µg/ml, ICC: 2.5 µg/ml, ELISA. However, the optimal conditions should be determined individually.|
|Other Names||Zinc finger protein 521, EHZF, Evi3|
|Formulation||100 µg (1 mg/ml) in 1X PBS containing 1 mg/ml BSA, 50% glycerol and less than 0.02% sodium azide, pH 7.4.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||ZNF521 Antibody (NT) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcription factor that can both act as an activator or a repressor depending on the context. Involved in BMP signaling and in the regulation of the immature compartment of the hematopoietic system. Associates with SMADs in response to BMP2 leading to activate transcription of BMP target genes. Acts as a transcriptional repressor via its interaction with EBF1, a transcription factor involved specification of B-cell lineage; this interaction preventing EBF1 to bind DNA and activate target genes.|
|Tissue Location||Predominantly expressed in hematopoietic cells. Present in organs and tissues that contain stem and progenitor cells, myeloid and/or lymphoid: placenta, spleen, lymph nodes, thymus, bone marrow and fetal liver. Within the hematopoietic system, it is abundant in CD34(+) cells but undetectable in mature peripheral blood leukocytes, and its levels rapidly decrease during the differentiation of CD34(+) cells in response to hemopoietins.|
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The zinc finger protein 521 (ZNF521) is a transcription factor containing an N-terminal transcriptional repressor motif and 30 zinc finger domains. It plays a role in both erythroid cell and osteoblast differentiation during development, inhibiting the activities of early B-cell factor 1 (EBF1) in erythroid cells and Runx2 in osteoblast precursors. ZFP521 binds to both Runx2 and histone deacetylase 3 (HDAC3), promotes their association and antagonizes Runx2 transcriptional activity in a HDAC3-dependent manner, thereby regulating osteoblast differentiation, skeletal development, and bone homeostasis.
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