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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ZNF521 Antibody (NT)   

ZNF521 Antibody (NT)

Rabbit Polyclonal Antibody

  • ICC - ZNF521 Antibody (NT) ABV10956
    Immunocytochemistry of ZNF521 (NT) in HeLa cells with ZNF521 (NT) antibody at 2.5μg/mL.
  • WB - ZNF521 Antibody (NT) ABV10956
    Western blot analysis of ZNF521 in HeLa cell lysate with ZNF521 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q96K83
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG1
Calculated MW 147866 Da
Additional Information
Gene ID 25925
Positive Control Western Blot: HeLa cell Lysate
IHC: HeLa cells
Application & Usage Western Blot: 1 µg/ml, ICC: 2.5 µg/ml, ELISA. However, the optimal conditions should be determined individually.
Other Names Zinc finger protein 521, EHZF, Evi3
Target/Specificity ZNF521
Antibody Form Liquid
Appearance Colorless liquid
Formulation 100 µg (1 mg/ml) in 1X PBS containing 1 mg/ml BSA, 50% glycerol and less than 0.02% sodium azide, pH 7.4.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsZNF521 Antibody (NT) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ZNF521
Synonyms EHZF, LIP3
Function Transcription factor that can both act as an activator or a repressor depending on the context. Involved in BMP signaling and in the regulation of the immature compartment of the hematopoietic system. Associates with SMADs in response to BMP2 leading to activate transcription of BMP target genes. Acts as a transcriptional repressor via its interaction with EBF1, a transcription factor involved specification of B-cell lineage; this interaction preventing EBF1 to bind DNA and activate target genes.
Cellular Location Nucleus.
Tissue Location Predominantly expressed in hematopoietic cells. Present in organs and tissues that contain stem and progenitor cells, myeloid and/or lymphoid: placenta, spleen, lymph nodes, thymus, bone marrow and fetal liver. Within the hematopoietic system, it is abundant in CD34(+) cells but undetectable in mature peripheral blood leukocytes, and its levels rapidly decrease during the differentiation of CD34(+) cells in response to hemopoietins.
Research Areas
Citations (0)

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The zinc finger protein 521 (ZNF521) is a transcription factor containing an N-terminal transcriptional repressor motif and 30 zinc finger domains. It plays a role in both erythroid cell and osteoblast differentiation during development, inhibiting the activities of early B-cell factor 1 (EBF1) in erythroid cells and Runx2 in osteoblast precursors. ZFP521 binds to both Runx2 and histone deacetylase 3 (HDAC3), promotes their association and antagonizes Runx2 transcriptional activity in a HDAC3-dependent manner, thereby regulating osteoblast differentiation, skeletal development, and bone homeostasis.

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$ 375.00
Cat# ABV10956
(40 western blots)
Availability: 5-7days
Bulk Size
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