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>   home   >   Products   >   Primary Antibodies   >   Protein G Antibody   

Protein G Antibody

Rabbit Polyclonal Antibody

     
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P19909
Other Accession CAA27638
Reactivity Human, Mouse, Rat, Goat, Cow, Sheep, Horse, Guinea Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Additional Information
Application & Usage Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Recombinant Protein G can be used as a positive control.
Other Names Immunoglobulin G-binding protein G, IgG-binding protein G, spg
Target/Specificity Protein G
Antibody Form Liquid
Appearance Colorless liquid
Formulation 100 µg (0.5 mg/ml) affinity purified rabbit anti-Protein G polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsProtein G Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name spg
Cellular Location Secreted, cell wall {ECO:0000255|PROSITE- ProRule:PRU00477}; Peptidoglycan-anchor {ECO:0000255|PROSITE- ProRule:PRU00477} EMBL; X06173; CAA29540.1; -; Genomic_DNA EMBL; Y00428; CAA68489.1; -; Genomic_DNA EMBL; X04015; CAA27638.1; -; Genomic_DNA PIR; S00128; S00128 PDB; 1FCC; X-ray; 3.20 A; C/D=372-427 PDB; 1FCL; NMR; -; A=372-427 PDB; 1FD6; NMR; -; A=372-427 PDB; 1GB4; NMR; -; A=372-427 PDB; 1GJS; NMR; -; A=254-299 PDB; 1GJT; NMR; -; A=254-299 PDB; 1IBX; NMR; -; B=- PDB; 1P7E; NMR; -; A=444-497 PDB; 1P7F; NMR; -; A=444-497 PDB; 1QKZ; X-ray; 1.95 A; A=368-430 PDB; 1UWX; X-ray; 2.20 A; A/B=368-430 PDB; 1ZXH; NMR; -; A=453-495 PDB; 2GI9; X-ray; 1.14 A; A=303-357 PDB; 2I2Y; NMR; -; A=304-357 PDB; 2I38; NMR; -; A=304-357 PDB; 2IGG; NMR; -; A=367-430 PDB; 2JSV; NMR; -; X=303-357 PDB; 2JU6; NMR; -; X=304-357 PDB; 2KHU; NMR; -; A=304-357 PDB; 2KHW; NMR; -; A=304-357 PDB; 2KN4; NMR; -; A=304-357 PDB; 2KQ4; NMR; -; X=303-357 PDB; 2KWD; NMR; -; A/B/C/D/E=304-357 PDB; 2LUM; NMR; -; A=444-497 PDB; 2OED; NMR; -; A=444-497 PDB; 2ON8; X-ray; 1.35 A; A=373-427 PDB; 2ONQ; X-ray; 1.70 A; A=375-427 PDB; 2QMT; X-ray; 1.05 A; A=303-357 PDB; 3FIL; X-ray; 0.88 A; A/B=303-357 PDB; 3UI3; X-ray; 2.80 A; A/B=304-357 PDB; 3V3X; X-ray; 2.00 A; A/B/C/D=304-357 PDB; 4OZA; X-ray; 2.20 A; A=302-357 PDB; 4OZB; X-ray; 1.80 A; A/B=302-357 PDB; 4OZC; X-ray; 2.30 A; A=302-357 PDB; 4WH4; X-ray; 2.20 A; A/B=304-357 PDB; 5BMG; X-ray; 2.20 A; A/B/C/D/E/F/G/H=304-357 PDB; 5BMH; X-ray; 1.60 A; A=304-357 PDB; 5BMI; X-ray; 2.50 A; A=304-357 PDB; 5HFY; X-ray; 1.95 A; A/B=302-357 PDB; 5HG2; X-ray; 1.80 A; A/B/C/D=302-357 PDB; 5HI1; X-ray; 2.15 A; A/B/C/D/E/F/G/H=302-357 PDBsum; 1FCC; - PDBsum; 1FCL; - PDBsum; 1FD6; - PDBsum; 1GB4; - PDBsum; 1GJS; - PDBsum; 1GJT; - PDBsum; 1IBX; - PDBsum; 1P7E; - PDBsum; 1P7F; - PDBsum; 1QKZ; - PDBsum; 1UWX; - PDBsum; 1ZXH; - PDBsum; 2GI9; - PDBsum; 2I2Y; - PDBsum; 2I38; - PDBsum; 2IGG; - PDBsum; 2JSV; - PDBsum; 2JU6; - PDBsum; 2KHU; - PDBsum; 2KHW; - PDBsum; 2KN4; - PDBsum; 2KQ4; - PDBsum; 2KWD; - PDBsum; 2LUM; - PDBsum; 2OED; - PDBsum; 2ON8; - PDBsum; 2ONQ; - PDBsum; 2QMT; - PDBsum; 3FIL; - PDBsum; 3UI3; - PDBsum; 3V3X; - PDBsum; 4OZA; - PDBsum; 4OZB; - PDBsum; 4OZC; - PDBsum; 4WH4; - PDBsum; 5BMG; - PDBsum; 5BMH; - PDBsum; 5BMI; - PDBsum; 5HFY; - PDBsum; 5HG2; - PDBsum; 5HI1; - ProteinModelPortal; P19909; - SMR; P19909; - MINT; MINT-1509283; - PRIDE; P19909; - EvolutionaryTrace; P19909; - GO; GO:0005618; C:cell wall; IEA:UniProtKB-SubCell GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW GO; GO:0016020; C:membrane; IEA:InterPro GO; GO:0009405; P:pathogenesis; IEA:InterPro InterPro; IPR019948; Gram-positive_anchor InterPro; IPR009063; Ig/albumin-bd InterPro; IPR000724; IgG-bd_B InterPro; IPR019950; M_anchor InterPro; IPR005877; YSIRK_signal_dom Pfam; PF00746; Gram_pos_anchor; 1 Pfam; PF01378; IgG_binding_B; 3 PRINTS; PR00015; GPOSANCHOR SUPFAM; SSF46997; SSF46997; 3 TIGRFAMs; TIGR01168; YSIRK_signal; 1 PROSITE; PS50847; GRAM_POS_ANCHORING; 1 1: Evidence at protein level; 3D-structure; Cell wall; IgG-binding protein; Peptidoglycan-anchor; Repeat; Secreted; Signal SIGNAL 1 33 CHAIN 34 562 Immunoglobulin G-binding protein G /FTId=PRO_0000005659 PROPEP 563 593 Removed by sortase. {ECO:0000255|PROSITE- ProRule:PRU00477} /FTId=PRO_0000005660 REPEAT 104 140 1-1 REPEAT 179 215 1-2 REPEAT 254 290 1-3 REPEAT 303 357 2-1 REPEAT 373 427 2-2 REGION 104 290 3 X 37 AA repeats REGION 303 427 2 X 55 AA repeats REGION 531 555 5 X 5 AA repeats of [DE]-D-A-K-K MOTIF 559 563 LPXTG sorting signal {ECO:0000255|PROSITE-ProRule:PRU00477} COMPBIAS 69 111 Ala-rich MOD_RES 562 562 Pentaglycyl murein peptidoglycan amidated threonine. {ECO:0000255|PROSITE- ProRule:PRU00477} HELIX 254 268 {ECO:0000244|PDB:1GJS} HELIX 272 279 {ECO:0000244|PDB:1GJS} HELIX 284 296 {ECO:0000244|PDB:1GJS} STRAND 303 309 {ECO:0000244|PDB:3FIL} STRAND 311 313 {ECO:0000244|PDB:2I2Y} STRAND 314 320 {ECO:0000244|PDB:3FIL} HELIX 324 337 {ECO:0000244|PDB:3FIL} STRAND 343 347 {ECO:0000244|PDB:3FIL} HELIX 348 350 {ECO:0000244|PDB:3FIL} STRAND 352 356 {ECO:0000244|PDB:3FIL} STRAND 373 379 {ECO:0000244|PDB:2ON8} STRAND 384 390 {ECO:0000244|PDB:2ON8} STRAND 391 393 {ECO:0000244|PDB:1FCL} HELIX 394 407 {ECO:0000244|PDB:2ON8} STRAND 413 417 {ECO:0000244|PDB:2ON8} TURN 418 421 {ECO:0000244|PDB:2ON8} STRAND 422 426 {ECO:0000244|PDB:2ON8} HELIX 431 440 {ECO:0000244|PDB:2KN4} STRAND 444 449 {ECO:0000244|PDB:1P7E} STRAND 454 460 {ECO:0000244|PDB:1P7E} HELIX 464 477 {ECO:0000244|PDB:1P7E} STRAND 483 487 {ECO:0000244|PDB:1P7E} TURN 488 491 {ECO:0000244|PDB:1P7E} STRAND 492 496 {ECO:0000244|PDB:1P7E} SEQUENCE 593 AA; 63292 MW; 048BAA760D5B2920 CRC64; MEKEKKVKYF LRKSAFGLAS VSAAFLVGST VFAVDSPIED TPIIRNGGEL TNLLGNSETT LALRNEESAT ADLTAAAVAD TVAAAAAENA GAAAWEAAAA ADALAKAKAD ALKEFNKYGV SDYYKNLINN AKTVEGVKDL QAQVVESAKK ARISEATDGL SDFLKSQTPA EDTVKSIELA EAKVLANREL DKYGVSDYHK NLINNAKTVE GVKDLQAQVV ESAKKARISE ATDGLSDFLK SQTPAEDTVK SIELAEAKVL ANRELDKYGV SDYYKNLINN AKTVEGVKAL IDEILAALPK TDTYKLILNG KTLKGETTTE AVDAATAEKV FKQYANDNGV DGEWTYDDAT KTFTVTEKPE VIDASELTPA VTTYKLVING KTLKGETTTE AVDAATAEKV FKQYANDNGV DGEWTYDDAT KTFTVTEKPE VIDASELTPA VTTYKLVING KTLKGETTTK AVDAETAEKA FKQYANDNGV DGVWTYDDAT KTFTVTEMVT EVPGDAPTEP EKPEASIPLV PLTPATPIAK DDAKKDDTKK EDAKKPEAKK EDAKKAETLP TTGEGSNPFF TAAALAVMAG AGALAVASKR KED
Citations (0)

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Background

Protein G binds to the constant region of many species of immunoglobulin G. Protein G can be used to detect, quantify and purify IgG antibodies and antibody/antigen complexes. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse.

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$ 89.00
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Cat# ABV10984-30
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