|Calculated MW||11927 Da|
|Application & Usage||Western blot (at 0.5-4 µg/ml). However, the optimal conditions should be determined individually.|
|Other Names||B-lymphocyte chemoattractant|
|Formulation||100 µg (0.5 mg/ml) Protein A purified rabbit anti-BLC polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||BLC Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Strongly chemotactic for B-lymphocytes, weakly for spleen monocytes and macrophages but no chemotactic activity for granulocytes. Binds to BLR1/CXCR5. May play a role in directing the migration of B-lymphocytes to follicles in secondary lymphoid organs.|
|Tissue Location||Found in spleen (B-cell-rich zone or follicles), Peyer patches (strongest within germinal centers and extending to the mantle zone) and lymph nodes (in reticular pattern in follicles)|
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Provided below are standard protocols that you may find useful for product applications.
BLC (B-lymphocyte chemoattractant) is a 12 kDa ELR-CXC chemokine. BLC showed high levels of expression in spleen, Peyer’s patches, and mesenteric lymph nodes. BLC is reported as the ligand of CXCR5. It attracts primarily B cells in vitro. Cell lines transfected with CXCR5 demonstrate both chemotaxis and calcium flux in response to BLC.
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