|Reactivity||Human, Mouse, Rat|
|Calculated MW||44341 Da|
|Positive Control||Jurkat cell lysate, mouse 3T3 cell lysate, and rat kidney tissue lysate|
|Application & Usage||The antibody can be used for Western blot analysis (0.5-4 µg/ml) and immunohistochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. Blocking peptide is available separately.|
|Other Names||Transforming growth factor beta-1, TGF-beta-1|
|Formulation||100 µg (0.5 mg/ml) affinity purified, rabbit anti-TGF-β1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||TGF-β1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Can promote either T- helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells.|
|Cellular Location||Secreted, extracellular space, extracellular matrix|
|Tissue Location||Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Colocalizes with ASPN in chondrocytes within OA lesions of articular cartilage.|
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Provided below are standard protocols that you may find useful for product applications.
TGF-β(transforming growth factor-beta) is a family of multifunctional proteins. TGF-β was originally identified for its ability to induce the growth of normal rodent fibroblast in soft agar. It is now known that TGF-β is a potent growth inhibitor of many normal and transformed cell lines. It regulates normal cell growth, development and tissue remodeling following injury. TGF-β is produced as latent high molecular weight complexes. The activation of latent TGF-β is an important step in the regulation of its action.
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