|Application ||WB, IF|
|Calculated MW||38568 Da|
|Application & Usage||Western blotting (1-4 µg/ml) and immunofluorescence (5-20 µg/ml). However, the optimal conditions should be determined individually. Other applications have not been determined. The antibody detects a 38-46 kDa band in samples of human, mouse and rat origins. Cross-reactivity to other species has not been determined.|
|Other Names||Alcohol dehydrogenase|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti- ADH polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||ADH Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Alcohol dehydrogenase with a preference for medium chain secondary alcohols, such as 2-butanol and isopropanol. Has very low activity with primary alcohols, such as ethanol. Under physiological conditions, the enzyme reduces aldehydes and 2- ketones to produce secondary alcohols. Is also active with acetaldehyde and propionaldehyde.|
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Provided below are standard protocols that you may find useful for product applications.
ADH (alcohol dehydrogenase) family of proteins metabolizes a variety of substances such as ethanol, retinal, other aliphatic alcohol, hydroxysteroids, and lipid peroxidation products. With the coenzyme NAD, ADH catalyzes the reversible conversion of organic alcohol to ketones or aldehydes. ADH plays a major role in ethanol metabolism.
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