|Calculated MW||21602 Da|
|Positive Control||Recombinant human VEGF-B|
|Application & Usage||Western Blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Recombinant human VEGF-B can be used as a positive control.|
|Other Names||Vascular endothelial growth factor B, VEGF-B, VEGF-related factor, VRF|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||VEGF-B Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.|
|Cellular Location||Secreted. Note=Secreted but remains associated to cells or to the extracellular matrix unless released by heparin|
|Tissue Location||Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas|
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Provided below are standard protocols that you may find useful for product applications.
VEGF-B, a member of the VEGF family, is a potent growth and angiogenic cytokine. It promotes DNA synthesis in endothelial cells, regulates angiogenesis and vascular permeability, and inhibits apoptosis in certain smooth muscle cells and neurons. VEGF-B is expressed in all tissues except the liver. It forms cell surfaced-associated disulfide linked homodimers and can form heterodimers with VEGF-A. There are two known isoforms, formed by alternative splicing, which have been designated VEGF-B167 and VEGF-B186. Both forms have identical amino-terminal sequences encoding a “cysteine knot” like structural motif, but differ in their carboxyl-terminal domains. Both VEGF-B isoforms signal only thro µgh the VEGFR1 receptor. Recombinant human VEGF-B is a 38.0 kDa disulfide-linked homodimeric protein consisting of two 167 amino acid polypeptide chains.
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