|Calculated MW||26152 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. Jurkat cell lysate can be used as a positive control. The antibody recognizes the 30 kDa human Galectin-3 in Western blot analysis with Jurkat cell lysate.|
|Other Names||Galectin3, Galectin 3, Galectin-3, Galactoside binding protein, GALBP, GAL3 , GAL 3, GAL-3, Galactose-specific lectin 3, Laminin-binding protein, MAC 2 antigen, MAC2, Lectin L-29, L-34 galactoside-binding lectin|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-human Galectin-3 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Galectin-3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.|
|Cellular Location||Cytoplasm. Nucleus. Secreted. Note=Secreted by a non-classical secretory pathway and associates with the cell surface|
|Tissue Location||A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages Expressed in fetal membranes.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Galectins are a family of soluble β-galactoside-binding animal lectins that modulate cell-to-cell and cell-to-ECM interactions, as well as apoptosis. Galectin-3 encodes a 30-35 kDa protein containing a single carbohydrate binding domain, which binds galactose-containing glycoconj µgates. Upregulated expression of Galectin-3 is involved in cancer progression and metastasis.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.