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>   home   >   Products   >   Primary Antibodies   >   Cancer   >   MART-1/Melan-A Antibody   

MART-1/Melan-A Antibody

Rabbit Polyclonal Antibody

  • WB - MART-1/Melan-A Antibody ABV11071
    Western blot analysis using recombinant human MART-1. Lane1: 10 ng; Lane 2: 50 ng
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q16655
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 13157 Da
Additional Information
Gene ID 2315
Application & Usage Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. Recombinant human MART-1 can be used as a positive control.
Other Names Melanoma antigen recognized by T-cells 1; MART-1; Protein Melan-A; Antigen SK29-AA; Antigen LB39-AA
Target/Specificity MART-1/Melan-A
Antibody Form Liquid
Appearance Colorless liquid
Formulation 100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsMART-1/Melan-A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms MART1
Function Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.
Cellular Location Endoplasmic reticulum membrane; Single-pass type III membrane protein. Golgi apparatus. Golgi apparatus, trans-Golgi network membrane. Melanosome. Note=Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post- Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation
Tissue Location Expression is restricted to melanoma and melanocyte cell lines and retina
Research Areas
Citations (0)

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MART-1 (also known as Melan-A) is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Six other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, Tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum.

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$ 245.00
$ 89.00
Cat# ABV11071
(40 western blots)
Availability: 5-7days
Bulk Size
Seasonal Special on Bulk Order
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