C5a Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | P06684 |
Reactivity | Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 188878 Da |
Gene ID | 15139 |
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Application & Usage | Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Recombinant mouse C5a can be used as a positive control. Other applications have not been determined. |
Other Names | complement C5a |
Target/Specificity | C5a |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | 100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal. |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | C5a Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | C5 |
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Synonyms | Hc |
Function | Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. |
Cellular Location | Secreted. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Mouse complement C5a is a 77-amino acid peptide generated during complement activation from the alpha-chain of complement C5. Mouse C5a shares 60% and 82% amino acid sequence identity to human and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (GPCR) (C5aR/CD88), inducing neutrophil chemotaxis and endothelial cell activation. It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.
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