|Calculated MW||188878 Da|
|Application & Usage||Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Recombinant mouse C5a can be used as a positive control. Other applications have not been determined.|
|Other Names||complement C5a|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||C5a Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.|
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Provided below are standard protocols that you may find useful for product applications.
Mouse complement C5a is a 77-amino acid peptide generated during complement activation from the alpha-chain of complement C5. Mouse C5a shares 60% and 82% amino acid sequence identity to human and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (GPCR) (C5aR/CD88), inducing neutrophil chemotaxis and endothelial cell activation. It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.
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