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C5a Antibody

Rabbit Polyclonal Antibody

     
  • WB - C5a Antibody ABV11077-30
    Western blot analysis of mC5a using recombinant mouse C5a. Lane 1: 20 ng; Lane 2: 50 ng
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  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P06684
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 188878 Da
Additional Information
Gene ID 15139
Application & Usage Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Recombinant mouse C5a can be used as a positive control. Other applications have not been determined.
Other Names complement C5a
Target/Specificity C5a
Antibody Form Liquid
Appearance Colorless liquid
Formulation 100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsC5a Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name C5
Synonyms Hc
Function Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.
Cellular Location Secreted.
Research Areas
Citations (0)
citation

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Background

Mouse complement C5a is a 77-amino acid peptide generated during complement activation from the alpha-chain of complement C5. Mouse C5a shares 60% and 82% amino acid sequence identity to human and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (GPCR) (C5aR/CD88), inducing neutrophil chemotaxis and endothelial cell activation. It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.

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Discontinued
Cat# ABV11077-30
Size:

Ordering Information

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