|Reactivity||Human, Mouse, Rat|
|Calculated MW||26809 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Recombinant adiponectin (4903-25) can be used as a positive control. The antibody detects the 30 kDa mouse adiponectin, and also cross-react in a lesser extent with human and rat adiponectin.|
|Other Names||ACDC , ACRP30 , Acrp30, AdipoQ|
|Formulation||50 µg (0.5 mg/ml) purified mouse anti-adiponectin monoclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Adiponectin Antibody (Clone MADI-1147) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||Acdc, Acrp30, Apm1|
|Function||Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.|
|Tissue Location||Synthesized exclusively by adipocytes and secreted into plasma|
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Provided below are standard protocols that you may find useful for product applications.
Adipocytes are highly active cells with potent autocrine, paracrine, and endocrine functions. Adiponectin is a serum protein produced and secreted exclusively by adipose tissues. Unlike insulin and leptin, adiponectin levels in plasma remain constant thro µghout the day and are not acutely affected by food intake.
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