|Application & Usage||Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Recombinant mCherry can be used as a positive control.|
|Other Names||red fluorescent protein mCherry, mCherry red fluorescent protein|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-mCherry polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||mCherry Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
mCherry is the second generation monomeric red fluorescent protein that have improved brightness and photostability. The recombinant mCherry is expressed and purified from transformed E. coli using a method that ensures high purity and maximal fluorescence intensity. mCherry protein is a 28.8 kDa monomer with 256 amino acid.
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