|Calculated MW||46794 Da|
|Positive Control||Western blot: Murine testis lysate|
|Application & Usage||Western blot: 2 µg/ml. However, the optimal conditions should be determined individually.|
|Formulation||50 µg of antibody in 100 µl PBS containing 0.02% sodium azide.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||DNA Methyltransferase 2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp).|
|Tissue Location||Highly expressed in thymus, testis, and at much lower levels in spleen, lung, brain, heart, kidney, liver, skeletal muscle and embryonic stem cells|
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Provided below are standard protocols that you may find useful for product applications.
Methylation of DNA at cytosine residues plays an important role in the regulation of gene expression, genomic imprinting, and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, three families of mammalian DNA methyltransferase genes have been identified which include DNMT1, DNMT2, and DNMT3. DNMT1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. DNMT2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. DNMT2 contains all the sequence motifs diagnostic of DNA (cytosine-5)-methyltransferases but appears to lack the large N-terminal regulatory domain common to other eukaryotic methyltransferases.
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