|Calculated MW||46794 Da|
|Positive Control||Western blot: DNMT2 transfected cell lysate|
|Application & Usage||Western blot: 1-3 µg/ml. However, the optimal conditions should be determined individually.|
|Formulation||50 µg of antibody in 100 µl PBS containing 0.2% gelatin and 0.05% sodium azide.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||DNA Methyltransferase 2 (Clone 102B1259.2) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp).|
|Tissue Location||Highly expressed in thymus, testis, and at much lower levels in spleen, lung, brain, heart, kidney, liver, skeletal muscle and embryonic stem cells|
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Provided below are standard protocols that you may find useful for product applications.
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. DNMT2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. DNMT2 contains all the sequence motifs diagnostic of DNA (cytosine-5)-methyltransferases but appears to lack the large N-terminal regulatory domain common to other eukaryotic methyltransferases.
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