|Reactivity||Human, Mouse, Monkey, Chimpanzee, Bovine|
|Calculated MW||92903 Da|
|Positive Control||Western blot: Jurkat cell lysate, NIH 3T3 cell lysate|
|Application & Usage||Western blot: 1-3 µg/ml. However, the optimal conditions should be determined individually.|
|Other Names||Lysine-specific histone demethylase 1A (BRAF35-HDAC complex protein BHC110) (Flavin-containing amine oxidase domain-containing protein 2)|
|Formulation||50 µg of antibody in 100 µl PBS containing 0.05% BSA and 0.05% sodium azide.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||LSD1 (aa 400-450) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||AOF2, KDM1, KIAA0601, LSD1|
|Function||Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr- 6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Effector of SNAI1-mediated transcription repression of E- cadherin/CDH1, CDN7 and KRT8. Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7.|
|Tissue Location||Ubiquitously expressed.|
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LSD1 the first known lysine-specific histone demethylase, is an 866 amino acid nuclear protein belonging to flavin monoamine oxidase family. It contains a SWIRM domain, a FAD-binding motif and an amine oxidase domain. This protein is ubiquitously expressed and is a component of several histone deacetylase complexes. LSD1 acts as a component of the CoREST and other transcriptional co-repressor complexes and also plays an important role in silencing neuronal-specific genes in non-neuronal cells. It is also known to demethylate Lys4 of histone H3, a specific tag for epigenetic transcriptional activation. Reports s µggest that that LSD1 plays an important role in stimulating androgen-receptor-dependent transcription converting oxygen to hydrogen peroxide (might use alternative electron acceptors). Along with nuclear FHL2, LSD1 serves as a novel biomarker predictive for prostate cancer.
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