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Histone H3 (phospho-Ser28) (Clone 117C826) Antibody

Mouse Monoclonal Antibody

     
  • WB - Histone H3 (phospho-Ser28) (Clone 117C826) Antibody ABV11116-50
    WB using Histone H3 mAb. Lane1:Untreated Jurkat cell lysate; Lane2:FAS-antibody treated Jurkat Cell lysate.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P68431
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype Mouse IgG
Clone Names 117C826
Calculated MW 15404 Da
Additional Information
Gene ID 8350;8351;8352;8353;8354;8355;8356;8357;8358;8968
Positive Control Western blot: Untreated Jurkat cell lysate, FAS-antibody treated Jurkat cell lysate
Application & Usage Western blot: 2 µg/ml. However, the optimal conditions should be determined individually.
Other Names PHH3, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/I, Histone H3/j, Histone H3/k, Histone H3/l
Target/Specificity Histone H3
Antibody Form Liquid
Appearance Colorless liquid
Formulation 50 µg of antibody in 100 µl PBS containing 0.05% sodium azide.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsHistone H3 (phospho-Ser28) (Clone 117C826) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name H3C1 (HGNC:4766)
Synonyms H3FA, HIST1H3A
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Cellular Location Nucleus. Chromosome.
Citations (0)
citation

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Background

The phosphorylation of histone H3 plays an important role in gene expression, chromatin remodeling, chromosome condensation, and cell division. Histone H3 phosphorylation may initiate at different phases of the cell division in different organisms, but metaphase chromosomes are always found to be heavily phosphorylated. H3 phosphorylation at serine 28 is coupled with mitotic chromosome condensation in diverse mammalian cell lines.

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Discontinued
Cat# ABV11116-50
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