|Calculated MW||15404 Da|
|Positive Control||Western blot: Untreated Jurkat cell lysate, FAS-antibody treated Jurkat cell lysate|
|Application & Usage||Western blot: 2 µg/ml. However, the optimal conditions should be determined individually.|
|Other Names||PHH3, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/I, Histone H3/j, Histone H3/k, Histone H3/l|
|Formulation||50 µg of antibody in 100 µl PBS containing 0.05% sodium azide.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Histone H3 (phospho-Ser28) (Clone 117C826) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.|
|Cellular Location||Nucleus. Chromosome.|
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Provided below are standard protocols that you may find useful for product applications.
The phosphorylation of histone H3 plays an important role in gene expression, chromatin remodeling, chromosome condensation, and cell division. Histone H3 phosphorylation may initiate at different phases of the cell division in different organisms, but metaphase chromosomes are always found to be heavily phosphorylated. H3 phosphorylation at serine 28 is coupled with mitotic chromosome condensation in diverse mammalian cell lines.
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