|Calculated MW||85271 Da|
|Positive Control||Western blot: Mouse spleen lysate, human spleen lysate|
|Application & Usage||Western blot: 1-2 µg/ml. However, the optimal conditions should be determined individually.|
|Other Names||Enhancer of zeste homolog 1 (Drosophila), ENX-2, ENX2, EZH1, Histone-lysine N-methyltransferase EZH1, KIAA0388|
|Formulation||50 µg of antibody in 100 µl PBS containing 0.05% BSA and 0.05% sodium azide.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||EZH1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH2-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.|
|Cellular Location||Nucleus. Note=Colocalizes with trimethylated 'Lys-27' of histone H3|
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EZH1 is a human homolog of the Drosophila gene Enhancer of zeste E(z), a member of the polycomb group of transcriptional repressors. In mice, EZH1 is a regulator of homeotic gene expression implicated in the assembly of repressive protein complexes in chromatin. The strong sequence conservation between species s µggests potential role for EZH1 in human development as a transcriptional regulator and as a component of protein complexes that stably maintain heterochromatin.
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