|Application ||WB, E, IP|
|Application & Usage||Western blot: 1:2000 – 1:5000, IP/ELISA: 1:1000. However, the optimal conditions should be determined individually.|
|Other Names||Methyl Lysine|
|Formulation||50 µg of antibody in 200 µl PBS, pH 7.0, containing 50% glycerol and 0.01% sodium azide.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Methyl Lysine (Biotin) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of lysine involves methylation. Lysine can be methylated once, twice, or three times by lysine methyltransferases. The transfer of methyl groups from S-adenosyl methionine to histones is catalyzed by enzymes known as histone methyltransferases. Histones which are methylated on certain residues can act epigenetically to repress or activate gene expression.
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