UCHL5 Antibody
Chicken Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | Q9Y5K5 |
Reactivity | Human |
Host | Chicken |
Clonality | Polyclonal |
Isotype | Chicken IgG |
Calculated MW | 37607 Da |
Gene ID | 51377 |
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Application & Usage | Western blot: Robust detection of 100 ng of recombinant protein was possible when antibody was used at a final concentration of 5 µg/mL |
Other Names | AD-019, CGI-70, Ubiquitin carboxyl-terminal hydrolase isozyme L5, Ubiquitin C-terminal hydrolase, UCH37, Ubiquitin thioesterase L5, UCH37, UCH-L5, (Ubiquitin C-terminal hydrolase L5 |
Target/Specificity | UCHL5 |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | 50 µg of antibody in PBS containing 10% glycerol |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | UCHL5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | UCHL5 |
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Synonyms | UCH37 |
Function | Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1. |
Cellular Location | Cytoplasm. Nucleus. Note=Associates with the proteasome 19S subunit in the cytoplasm. Associates with the INO80 complex in the nucleus |
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Background
Protein ubiquitination and deubiquitination are reversible processes catalyzed by ubiquitinating enzymes (UBEs) and deubiquitinating enzymes (DUBs). DUBs are categorized into 5 subfamilies: USP, UCH, OTU, MJD, and JAMM. UCHL1, UCHL3, UCHL5/UCH37, and BRCA-1-associated protein-1 (BAP1) belong to the UCH family of DUBs, which all possess a conserved catalytic domain (UCH domain) of about 230 amino acids. UCHL5 and BAP1 have unique extended C-terminal tails. UCHL5 is the only ubiquitin carboxy-terminal hydrolase (UCH)-family protease that is associated with mammalian proteasomes. It is a protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1. It is responsible for the ubiquitin isopeptidase activity in the PA700 (19S) proteasome regulatory complex.
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