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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   USP5 Antibody   

USP5 Antibody

Chicken Polyclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P45974
Reactivity Human
Host Chicken
Clonality Polyclonal
Isotype Chicken IgG
Calculated MW 95786 Da
Additional Information
Gene ID 8078
Application & Usage Western blot: Robust detection of 100 ng of recombinant protein was possible when antibody was used at a final concentration of 5 µg/mL
Other Names Deubiquitinating enzyme 5, Isopeptidase T, IsoT, ISOT, Ubiquitin carboxyl-terminal hydrolase 5, Ubiquitin-specific-processing protease 5, Ubiquitin thioesterase 5, Ubiquitin-specific-processing protease 5.
Target/Specificity USP5
Antibody Form Liquid
Appearance Colorless liquid
Formulation 50 µg of antibody in PBS containing 10% glycerol
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsUSP5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name USP5
Synonyms ISOT
Function Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys- 63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.
Research Areas
Citations (0)

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The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP5 (ubiquitin specific peptidase 5), also known as ISOT (isopeptidase T), is a 858 amino acid zinc-binding deubiquitinating enzyme that participates in the Ub pathway. A member of the peptidase C19 family, the catalytic activity of USP5 involves a combination of the ubiquitin carboxyl-terminal thiolester and water to produce ubiquitin and a thiol. USP5 contains two UBA domains and one UBP-type zinc finger. USP5 is responsible for disassembling unanchored polyubiquitin chains in the cell. Full length USP5 mediated cleavage of Ub-PLA2 is significantly enhanced is enhanced by low concentrations of mono ubiquitin.

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$ 145.00
Cat# ABV11126
(40 western blots)
Availability: 5-7days
Bulk Size
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