|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||88207 Da|
|Positive Control||Western Blot: HEK-293 & HeLa cell lysates|
|Application & Usage||Western blot: 1:200 – 1:500 dilution, IHC: 1:50 - 1:100 dilution. However, the optimal conditions should be determined individually.|
|Other Names||USP1, UBP|
|Formulation||100 µg of antibody in 100 µl PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||USP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2 (PubMed:15694335). Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA (PubMed:16531995). Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity (PubMed:18082604, PubMed:26388029).|
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Provided below are standard protocols that you may find useful for product applications.
Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzymes (DUB) action. There are five DUB subfamilies including the USP, UCH, OTU, MJD, and JAMM enzymes. USP1 belongs to the USP subfamily and is regulated in a cell cycle dependent manner by both transcriptional and ubiquitinproteosomal mechanisms. USP1 is a nuclear protein and localizes to chromatin where it is specifically associated with FANCD2. USP1 deubiquitinates monoubiquitinated FANCD2, which plays an important role in DNA damage repair and Chk1 protein stability. USP1 deubiquitinates monoubiquitinated PCNA, thereby negatively regulating PCNAmediated translation synthesis (TLS) during DNA repair. USP1 interaction with UAF1, a WD40 repeat containing protein, leads to formation of an activated USP1/UAF1 complex, which is required for the deubiquitinase activity of USP1.
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