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USP25 Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | Q9UHP3 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 122218 Da |
| Gene ID | 29761 |
|---|---|
| Positive Control | Western Blot: BxPC-3 cell lysate |
| Application & Usage | Western blot: 1:500 – 1:1000 dilution. However, the optimal conditions should be determined individually. |
| Other Names | USP25, USP21 |
| Target/Specificity | USP25 |
| Antibody Form | Liquid |
| Appearance | Colorless liquid |
| Formulation | 100 µg of antibody in 100 µl PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3 |
| Handling | The antibody solution should be gently mixed before use. |
| Reconstitution & Storage | -20 °C |
| Background Descriptions | |
| Precautions | USP25 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | USP25 |
|---|---|
| Synonyms | USP21 |
| Function | Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions in various biological processes including inflammation, immune response (PubMed:29518389, PubMed:37683630). Modulates the Wnt/beta-catenin pathway by deubiquitinating and stabilizing tankyrases TNKS1 and TNKS2 (PubMed:28619731, PubMed:30926243). Regulates KEAP1-NRF2 axis in the defense against oxidative assaults by deubiquitinating KEAP1 and protecting it from degradation leading to degradation of the NRF2 transcription factor that is responsible for mounting an anti-oxidation gene expression program (PubMed:37339955). Positively regulates RNA virus-induced innate signaling by interacting with and deubiquitinating ERLIN1 and ERLIN2 (PubMed:37683630). In turn, restricts virus production by regulating cholesterol biosynthetic flux (PubMed:37683630). Acts as a negative regulator of interleukin-17- mediated signaling and inflammation through the removal of 'Lys-63'- linked ubiquitination of TRAF5 and TRAF6 (PubMed:23042150). Prevents the ubiquitination and degradation of TRAF3 to reduce the phosphorylation levels of JNK and P38, the secretion of IL-1B and to induce endotoxin tolerance (PubMed:30579117). |
| Cellular Location | Cytoplasm |
| Tissue Location | Isoform USP25a is found in most adult and fetal tissues; expression is moderately high in testis, pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, but very low in peripheral blood, colon, small intestine, ovary, prostate, thymus and spleen. Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzymes (DUB) action. There are five DUB subfamilies including the USP, UCH, OTU, MJD, and JAMM enzymes. USP25 (ubiquitin specific peptidase 25), also known as USP21, is a 1087 amino acid member of the peptidase C19 family. Expressed at high amounts in pancreas, kidney, skeletal muscle, testis, liver, heart, brain and lung, USP25 associates with the 26S proteasome and functions as a deubiquitinating enzyme in the Ub proteolytic pathway. Due to alternative splicing events, USP25 is expressed as two short, ubiquitously expressed isoforms and one long, muscle specific isoform. The long isoform of USP25 is upregulated in myogenesis and is implicated in the pathogenesis of severe myopathies.
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