|Reactivity||Human, Mouse, Rat|
|Calculated MW||122218 Da|
|Positive Control||Western Blot: BxPC-3 cell lysate|
|Application & Usage||Western blot: 1:500 – 1:1000 dilution. However, the optimal conditions should be determined individually.|
|Other Names||USP25, USP21|
|Formulation||100 µg of antibody in 100 µl PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||USP25 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains.|
|Tissue Location||Isoform USP25a is found in most adult and fetal tissues; expression is moderately high in testis, pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, but very low in peripheral blood, colon, small intestine, ovary, prostate, thymus and spleen. Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific.|
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Provided below are standard protocols that you may find useful for product applications.
Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzymes (DUB) action. There are five DUB subfamilies including the USP, UCH, OTU, MJD, and JAMM enzymes. USP25 (ubiquitin specific peptidase 25), also known as USP21, is a 1087 amino acid member of the peptidase C19 family. Expressed at high amounts in pancreas, kidney, skeletal muscle, testis, liver, heart, brain and lung, USP25 associates with the 26S proteasome and functions as a deubiquitinating enzyme in the Ub proteolytic pathway. Due to alternative splicing events, USP25 is expressed as two short, ubiquitously expressed isoforms and one long, muscle specific isoform. The long isoform of USP25 is upregulated in myogenesis and is implicated in the pathogenesis of severe myopathies.
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