|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||132370 Da|
|Positive Control||Western Blot: Various cell lysates|
|Application & Usage||Western blot: 1:500 – 1:2000, IHC: 1:50 – 1:200.|
|Other Names||BAT8, C6orf30, DKFZp686H08213, FLJ35547, G9A, KMT1C, NG36, NG3|
|Formulation||100 µg of antibody in 100 µl PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||EHMT2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||BAT8, C6orf30, G9A, KMT1C, NG36|
|Function||Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys- 373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.|
|Cellular Location||Nucleus. Chromosome. Note=Associates with euchromatic regions. Does not associate with heterochromatin|
|Tissue Location||Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow|
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Provided below are standard protocols that you may find useful for product applications.
G9a, also known as Euchromatic histonelysine Nmethyltransferase 2 (EHMT2), is a member of a family of histone lysine methyltransferases. Recombinant G9a can mono, di and trimethylate histone H3 on Lys9 and Lys27 in vitro. However, in vivo G9a forms a complex with GLP, a G9arelated histone methyltransferase, and together these proteins function as the major euchromatic histone H3 Lys9 mono and dimethyltransferases, creating transcriptionally repressive marks that facilitate gene silencing. G9a methylates itself on Lys165, a modification that regulates the association of HP1 repressor proteins with the G9a/GLP complex. The G9a/GLP complex also contains Wiz, a zinc finger protein that is required for G9a/GLP heterodimerization and complex stability. Wiz contains two CtBP corepressor binding sites, which mediate the association of the G9a/GLP with the CtBP corepressor complex. In addition, G9a and GLP are components of other large transcriptional corepressor complexes, such as those involving E2F6 and CDP/cut. G9a interacts with DNMT1, and both proteins are required for methylation of DNA and histone H3 (Lys9) at replication foci, providing a functional link between histone H3 Lys9 and CpG methylation during DNA replication. G9a activity is critical for meiotic prophase progression, as mutant mice deficient in germ line G9a show a large loss of mature gametes. In addition, G9a facilitates increased global levels of dimethyl histone H3 (Lys9) during hypoxic stress and increased G9a expression is associated with hepatocellular carcinoma.
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