|Application ||WB, IP|
|Isotype||Mouse IgG 1|
|Calculated MW||56507 Da|
|Positive Control||WB analysis of cell lysates|
|Application & Usage||Western blot: 1:1000, IP: 1-2 µl.|
|Other Names||Thioredoxin Reductase, GRIM-12, MGC9145, TR, TRXR2.|
|Target/Specificity||Thioredoxin Reductase 2|
|Formulation||100 µl of antibody in HEPES with 0.15 M NaCl, 0.01 % BSA, 0.03 % sodium azide, and 50 % glycerol|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Thioredoxin Reductase 2 Antibody (7B2) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in the control of reactive oxygen species levels and the regulation of mitochondrial redox homeostasis (PubMed:24601690). Maintains thioredoxin in a reduced state. May play a role in redox-regulated cell signaling.|
|Tissue Location||Highly expressed in the prostate, ovary, liver, testis, uterus, colon and small intestine. Intermediate levels in brain, skeletal muscle, heart and spleen. Low levels in placenta, pancreas, thymus and peripheral blood leukocytes According to PubMed:10608886, high levels in kidney, whereas according to PubMed:9923614, levels are low. High expression is observed in the adrenal cortex (PubMed:24601690)|
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Provided below are standard protocols that you may find useful for product applications.
The mammalian thioredoxin reductases (TrxRs) are a family of seleno-cysteine containing pyridine nucleotide-disulfide oxido-reductases. All the mammalian TrxRs are homologous to glutathione reductase with respect to primary structure including the conserved redox catalytic site (-Cys-Val-Asn-Val-Gly-Cys-) but distinctively with a C-terminal extension containing a catalytically active penultimate seleno-cysteine (SeCys) residue in the conserved sequence(-Gly-Cys-SeCys-Gly). TrxR is homodimeric protein in which each monomer includes an FAD prosthetic group, a NADPH binding site and a redox catalytic site. Electrons are transferred from NADPH via FAD and the active-site disulfide to C-terminal SeCys-containing redox center, which then reduces the substrate like thioredoxin. The members of TrxR family are 55 – 58 kDa in molecular size and composed of three isoforms including cytosolic TrxR1, mitochondrial TrxR2, and TrxR3, known as Trx and GSSG reductase (TGR). TrxR plays a key role in protection of cells against oxidative stress and redox-regulatory mechanism of transcription factors and various biological phenomena. TrxR2 maintains thioredoxin in a reduced state. Implicated in the defenses against oxidative stress. May play a role in redox-regulated cell signaling.
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