|Reactivity||Human, Mouse, Rat|
|Calculated MW||48848 Da|
|Positive Control||Western Blot: Rat kidney lysate|
|Application & Usage||Western blotting (0.5-4 µg/ml), Immunohistochemistry (20-30 µg/ml)|
|Other Names||HDAC3, SMAP45 , HD3 , RPD3 , RPD3-2|
|Formulation||100 µg (0.5 mg/ml) polyclonal antibody in PBS pH 7.2, containing 30% glycerol, 0.5% BSA and 0.01% thimerosal|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||HDAC3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Participates in the BCL6 transcriptional repressor activity by deacetylating the H3 'Lys- 27' (H3K27) on enhancer elements, antagonizing EP300 acetyltransferase activity and repressing proximal gene expression. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation (PubMed:21444723, PubMed:23911289). Contributes, together with XBP1 isoform 1, to the activation of NFE2L2-mediated HMOX1 transcription factor gene expression in a PI(3)K/mTORC2/Akt-dependent signaling pathway leading to endothelial cell (EC) survival under disturbed flow/oxidative stress (PubMed:25190803).|
|Cellular Location||Nucleus. Cytoplasm. Cytoplasm, cytosol. Note=Colocalizes with XBP1 and AKT1 in the cytoplasm (PubMed:25190803). Predominantly expressed in the nucleus in the presence of CCAR2.|
|Tissue Location||Widely expressed.|
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Provided below are standard protocols that you may find useful for product applications.
HDAC 3 is a member of the class I mammalian histone deacetylase family, which plays an important role in modulating the eukaryotic chromatin structure. Human HDAC 3 is composed of 428 amino acid residues. HDAC 3 interacts with a growing number of transcriptional factors including SMRT and N-CoR. The interacting complexes bind to specific regions of chromatin and regulate gene transcription in these regions.
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