|Reactivity||Human, Mouse, Rat|
|Calculated MW||20517 Da|
|Positive Control||Western Blot: Jurkat cell lysate|
|Application & Usage||Western blot: 1:200|
|Other Names||CSIF, IL-10, IL10A, TGIF, B-TCGF, GVHDS, MGC126450, MGC126451, RP11-262N9.1, Interleukin-10|
|Formulation||100 µg or 30 µg (0.5 mg/ml) of antibody in PBS containing 0.01 % BSA, 0.01 % thimerosal, and 50 % glycerol, pH7.2|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||IL-10 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.|
|Tissue Location||Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types|
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Provided below are standard protocols that you may find useful for product applications.
IL-10 (Interleukin-10), originally known as Cytokine Synthesis Inhibitory Factor (CSIF), is an 20.5 kDa protein containing 161-178 amino acid residues. IL10 is produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.
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