PSGL-1 Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
---|---|
Primary Accession | Q14242 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 43201 Da |
Gene ID | 6404 |
---|---|
Positive Control | Western blot: Jurkat Cell lysate |
Application & Usage | Western blot: 1-4 µg/ml. |
Other Names | PSGL, CD162, SELPLG, CLA, CD162, PSGL1, Selectin P ligand. |
Target/Specificity | PSGL-1 |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | 100 µg or 30 µg (0.5 mg/ml) of antibody in PBS pH 7.2 containing 0.01 % BSA, 0.01 % thimerosal, and 50 % glycerol. |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | PSGL-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | SELPLG |
---|---|
Function | A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture. |
Cellular Location | Membrane; Single-pass type I membrane protein. |
Tissue Location | Expressed on neutrophils, monocytes and most lymphocytes |
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Provided below are standard protocols that you may find useful for product applications.
Background
PSGL-1 (P-Selectin glycoprotein ligand, also designated CD162), exists as a disulfide-linked homodimer. PSGL-1 is a type 1 membrane protein that localizes on the tips of microvilli of leukocytes. Its extracellular domain is rich in serines, threonines and prolines, and includes a series of 15 and 16 decameric repeats in HL-60 and U-937 cells, and human leukocytes, respectively. Although PSGL-1 appears to be the sole receptor for P-Selectin on human hematopoietic cells, it also interacts with E-Selectin through a unique binding site. In order to bind PSGL-1 to either E-Selectin or P-Selectin, PSGL-1 must be sialylated and fucosylated. PSLG-1 is a mucin-like molecule, much like leukosialin (CD43), CD164 and CD34. These proteins belong to an emerging family of cell adhesion receptors called sialomucins, which transduce negative signals in hematopoietic cells.
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