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NNMT Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | P40261 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 29574 Da |
| Gene ID | 4837 |
|---|---|
| Positive Control | Western Blot: Recombinant protein |
| Application & Usage | Western blot: 1-4 µg/ml. |
| Other Names | Nicotinamide N-methyltransferase |
| Target/Specificity | NNMT |
| Antibody Form | Liquid |
| Appearance | Colorless liquid |
| Formulation | 100 µg (0.5 mg/ml) of antibody in PBS pH 7.2 containing 0.01 % BSA, 0.01 % thimerosal, and 50 % glycerol. |
| Handling | The antibody solution should be gently mixed before use. |
| Reconstitution & Storage | -20 °C |
| Background Descriptions | |
| Precautions | NNMT Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NNMT {ECO:0000303|PubMed:23455543} |
|---|---|
| Function | Catalyzes the N-methylation of nicotinamide using the universal methyl donor S-adenosyl-L-methionine to form N1- methylnicotinamide and S-adenosyl-L-homocysteine, a predominant nicotinamide/vitamin B3 clearance pathway (PubMed:8182091, PubMed:21823666, PubMed:23455543). Plays a central role in regulating cellular methylation potential, by consuming S-adenosyl-L-methionine and limiting its availability for other methyltransferases. Actively mediates genome-wide epigenetic and transcriptional changes through hypomethylation of repressive chromatin marks, such as H3K27me3 (PubMed:26571212, PubMed:23455543, PubMed:31043742). In a developmental context, contributes to low levels of the repressive histone marks that characterize pluripotent embryonic stem cell pre-implantation state (PubMed:26571212). Acts as a metabolic regulator primarily on white adipose tissue energy expenditure as well as hepatic gluconeogenesis and cholesterol biosynthesis. In white adipocytes, regulates polyamine flux by consuming S-adenosyl-L-methionine which provides for propylamine group in polyamine biosynthesis, whereas by consuming nicotinamide controls NAD(+) levels through the salvage pathway (By similarity). Via its product N1-methylnicotinamide regulates protein acetylation in hepatocytes, by repressing the ubiquitination and increasing the stability of SIRT1 deacetylase (By similarity). Can also N-methylate other pyridines structurally related to nicotinamide and play a role in xenobiotic detoxification (PubMed:30044909). |
| Cellular Location | Cytoplasm. |
| Tissue Location | Predominantly expressed in the liver. A lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. Not detected in the brain or pancreas |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. NNMT is an important cytosolic methyltransferase catalyzing the N-methylation of nicotinamide, pyridines and structural analogs, playing a crucial role in the biotransformation and detoxification of many xenobiotic compounds. In the N-methylation process, NNMT uses S-adenosyl methionine as the methyl donor and nicotinamide as methyl acceptor. NNMT is mostly expressed in the liver, and a lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. It may also play an important role in regulating biological processes related to N-methyl nicotinamide such as anti-inflammatory, anti-thrombotic, vasoprotective, and gastroprotective properties. Increased NNMT activity was reported in many kinds of tumors. NNMT is a potential biomarker and therapeutic target in cancer diagnosis and treatment. NNMT serum levels have significance in the premature detection and in the management of patients with colorectal cancer.
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