|Calculated MW||58951 Da|
|Positive Control||Western Blot: Recombinant protein|
|Application & Usage||Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects the proform (~55 kDa), intermediates (30 kDa and 34 kDa), and cleaved form (~20 kDa) of caspase-10 on SDS-PAGE immunoblots. |
|Other Names||FAS-associated death domain protein interleukin-1B-converting enzyme 2, FLICE2, ICE like Apoptotic Protease 4|
|Formulation||100 µg (0.5 mg/ml) of Caspase-10 antibody in PBS pH 7.2, 0.01 % BSA, 0.01 % thimerosal, and 50 % glycerol.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Caspase-10 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase- 3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC.|
|Tissue Location||Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon|
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Provided below are standard protocols that you may find useful for product applications.
Caspase family of cysteine proteases play a key role in apoptosis. Caspase-10 is expressed in many tissues and cell lines, and has been identified as caspase-10/a (short form) and caspase-10/b (long form), respectively. Similar as other caspases, caspase-10 is also synthesized as an inactive pro-enzyme that is processed in cells undergoing apoptosis. Activated caspase-10 activates caspase-3,-4,-6,-7,-8, and –9, which are then involved in proteolytic cleavage of many cellular proteins. Caspase-10 has been shown involving in the Fas and TNFR1 induced apoptosis.
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