Procathepsin B Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
---|---|
Primary Accession | P07858 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 37822 Da |
Gene ID | 1508 |
---|---|
Positive Control | Western Blot: Jurkat cell lysate, 3T3 cell lysate, rat kidney lysate, recombinant protein |
Application & Usage | Western blot: 1-4 µg |
Other Names | CTSB, CPSB |
Target/Specificity | Procathepsin B |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | 100 µg (0.5 mg/ml) of antibody in PBS pH 7.2 containing 0.01 % BSA, 0.01 % thimerosal, and 50 % glycerol. |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | Procathepsin B Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | CTSB |
---|---|
Synonyms | CPSB |
Function | Thiol protease which is believed to participate in intracellular degradation and turnover of proteins (PubMed:12220505). Cleaves matrix extracellular phosphoglycoprotein MEPE (PubMed:12220505). Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen (By similarity). Has also been implicated in tumor invasion and metastasis (PubMed:3972105). |
Cellular Location | Lysosome. Melanosome. Secreted, extracellular space {ECO:0000250|UniProtKB:A1E295}. Apical cell membrane {ECO:0000250|UniProtKB:P10605}; Peripheral membrane protein {ECO:0000250|UniProtKB:P10605}; Extracellular side {ECO:0000250|UniProtKB:P10605}. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065) Localizes to the lumen of thyroid follicles and to the apical membrane of thyroid epithelial cells (By similarity) {ECO:0000250|UniProtKB:P10605, ECO:0000269|PubMed:17081065} |
Tissue Location | Expressed in the stratum spinosum of the epidermis. Weak expression is detected in the stratum granulosum |
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Provided below are standard protocols that you may find useful for product applications.
Background
Cathepsin B is a normal lysosomal cysteine proteinase that is expressed in all cells. It is synthesized as an inactive 43 kDa pro-enzyme which, by removal of a 62 amino acid propeptide, is activated to the single-chain form of 31 kDa or the two-chain form (25 and 5 kDa subunits). Matured cathepsin B is normally localized in the lysosomes where it functions in protein turnover. Cathepsin B is also shown capable of degrading extracellular matrix proteins. Elevated levels of cathepsin B correlate with malignancy suggesting that this enzyme may be a useful prognostic marker for several types of human cancers.
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