|Reactivity||Human, Mouse, Rat|
|Calculated MW||46312 Da|
|Positive Control||Western Blot: Jurkat cell lysate, 3T3 cell lysate, mouse muscle lysate, rat kidney lysate, recombinant protein|
|Application & Usage||Western blot: 1-4 µg|
|Other Names||Pigment epithelium-derived factor, PEDF, Serpin-F1, SerpinF1, EPC-1, EPC1, PIG35|
|Formulation||100 µg (0.5 mg/ml) of antibody in PBS pH 7.2 containing 0.01 % BSA, 0.01 % thimerosal, and 50 % glycerol.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||PEDF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.|
|Cellular Location||Secreted. Melanosome. Note=Enriched in stage I melanosomes|
|Tissue Location||Retinal pigment epithelial cells and blood plasma.|
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PEDF is a non-inhibitory serpin with neurotrophic, anti-angiogenic, and anti-tumorigenic properties. PEDF is a 50 kDa glycoprotein created and secreted in many tissues all the way through the body. A key component of the anti-angiogenic action of PEDF is the induction of apoptosis in proliferating endothelial cells. Additionally, PEDF is capable to inhibit the activity of angiogenic factors such as VEGF and FGF-2. The recognition of a lipase-linked cell membrane receptor for PEDF (PEDF-R) that binds to PEDF with high affinity should facilitate further elucidation of the underlying mechanisms of this pluripotent serpin. The unique range of PEDF activities associate it as a potential therapeutic agent for the treatment of vasculature related neurodegenerative diseases such as age-related macular degeneration (AMD) and proliferative diabetic retinopathy (PDR).
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