|Reactivity||Human, Mouse, Rat|
|Calculated MW||28999 Da|
|Positive Control||Western Blot: mouse muscle lysate, rat kidney lysate, recombinant protein|
|Application & Usage||Western blot: 1-4 µg|
|Other Names||CTL tryptase, Cytotoxic T-lymphocyte proteinase 1, Fragmentin-1, Granzyme-1, Hanukkah factor, H factor, HF|
|Formulation||100 µg (0.5 mg/ml) of antibody in PBS pH 7.2 containing 0.01 % BSA, 0.01 % thimerosal, and 50 % glycerol.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Granzyme A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent cell death with morphological features of apoptosis when delivered into the target cell through the immunological synapse. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Cleaves the nucleosome assembly protein SET after 'Lys-189', which disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA.|
|Cellular Location||Isoform alpha: Secreted. Cytoplasmic granule|
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Provided below are standard protocols that you may find useful for product applications.
Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells. Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein. Granzyme A is the most abundant serine protease in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme A has a specific function in CTL and NK cells. It induces caspase-independent cell death when introduced into target cells by perforin. Granzyme B has the strongest apoptotic activity of all the granzymes as a result of its caspase-like ability to cleave substrates at aspartic acid residues thereby activating procaspases directly and cleaving downstream caspase substrates
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