|Calculated MW||45640 Da|
|Positive Control||Western Blot: Jurkat cell lysate, mouse muscle lysate, recombinant protein|
|Application & Usage||Western blot: 1-4 µg|
|Other Names||CASP-1, Interleukin-1 beta convertase, IL-1BC, Interleukin-1 beta-converting enzyme, ICE, IL-1 beta-converting enzyme|
|Formulation||100 µg (0.5 mg/ml) of antibody in PBS pH 7.2, 0.01 % BSA, 0.03 % ProClin®, and 50 % glycerol.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Caspase 1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.|
|Tissue Location||High level expression seen in spleen and lung, low level expression seen in brain, heart, liver, kidney, testis and skeletal muscle|
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Provided below are standard protocols that you may find useful for product applications.
Caspase-1 (also known as ICE) is a prototypical member of the caspase-family of cysteine proteases. Caspase-1 exists in cells as an inactive 45 kDa proenzyme. The pro-enzyme is matured by proteolysis to yield large (20 kDa) and small (10 kDa) subunits. The active caspase-1 is a heterotetramer consisting of two large and two small subunits. To date the regulatory mechanism of caspase-1 activation and the role of caspase-1 in apoptosis are poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive proenzyme form.
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