|Application ||WB, E|
|Calculated MW||68420 Da|
|Positive Control||Western blot: MCF7 Cell lysate|
|Application & Usage||Western blot: ~1:1000 - 2000, ELISA.|
|Other Names||Tyrosyl-DNA phosphodiesterase 1, TDP1 protein, TDP1.|
|Formulation||Supplied in PBS with 0.1% sodium azide.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||TDP1 Antibody (Clone AT1F2) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. Has low 3'exonuclease activity and can remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate.|
|Cellular Location||Nucleus. Cytoplasm|
|Tissue Location||Ubiquitously expressed. Similar expression throughout the central nervous system (whole brain, amygdala, caudate nucleus, cerebellum, cerebral cortex, frontal lobe, hippocampus, medulla oblongata, occipital lobe, putamen, substantia nigra, temporal lobe, thalamus, nucleus accumbens and spinal cord) and increased expression in testis and thymus|
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Provided below are standard protocols that you may find useful for product applications.
Tyrosyl-DNA phosphodiesterase (TDP1) is a DNA repair enzyme that catalyzes the hydrolysis of a phophodiester bond between a tyrosine residue and a DNA 3' phosphate. This protein is a member of the phospholipase D (PLD) superfamily and contains two PLD phosphodiesterase domains. Mutations in TDP1 are associated with the disease spino-cerebellar ataxia with axonal neuropathy (SCAN1) by interfering with DNA transcription or by inducting apoptosis in postmitotic neurons.
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