|Application ||WB, IHC|
|Calculated MW||98989 Da|
|Positive Control||Western blot: HeLa and mouse brain lysate, IHC: human liver tissue|
|Application & Usage||Western blot: ~1:1000, IHC: ~1:10-1:50|
|Other Names||AR; DHTR; NR3C4; Androgen receptor; Dihydrotestosterone receptor; Nuclear receptor subfamily 3 group C member 4|
|Formulation||100 µl of antibody in PBS with 0.09% (W/V) sodium azide|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Androgen Receptor Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.|
|Cellular Location||Nucleus. Cytoplasm. Note=Predominantly cytoplasmic in unligated form but translocates to the nucleus upon ligand-binding. Can also translocate to the nucleus in unligated form in the presence of GNB2L1|
|Tissue Location||Isoform 2 is mainly expressed in heart and skeletal muscle.|
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Provided below are standard protocols that you may find useful for product applications.
Androgens exhibit a wide range of effects on the development, maintenance and regulation of male phenotype and reproductive physiology in males. The androgen receptor (AR) is a member of the steroid superfamily of ligand-dependent transcription factors. ARs bind active testosterone (T) and dihydrotestosterone (DHT). The rates of association and dissociation of T are about 3 times more rapid than those of DHT. This difference in binding kinetics may account for the different physiological effects of T and DHT. Androgen binding results in an at least 6-fold increase in androgen receptor stability.
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