Androgen Receptor Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC |
---|---|
Primary Accession | P10275 |
Reactivity | Human, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 99188 Da |
Gene ID | 367 |
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Positive Control | Western blot: HeLa and mouse brain lysate, IHC: human liver tissue |
Application & Usage | Western blot: ~1:1000, IHC: ~1:10-1:50 |
Other Names | AR; DHTR; NR3C4; Androgen receptor; Dihydrotestosterone receptor; Nuclear receptor subfamily 3 group C member 4 |
Target/Specificity | AR |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | 100 µl of antibody in PBS with 0.09% (W/V) sodium azide |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | Androgen Receptor Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | AR |
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Synonyms | DHTR, NR3C4 |
Function | Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues (PubMed:19022849). Transcription factor activity is modulated by bound coactivator and corepressor proteins like ZBTB7A that recruits NCOR1 and NCOR2 to the androgen response elements/ARE on target genes, negatively regulating androgen receptor signaling and androgen-induced cell proliferation (PubMed:20812024). Transcription activation is also down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3. |
Cellular Location | Nucleus. Cytoplasm Note=Detected at the promoter of target genes (PubMed:25091737) Predominantly cytoplasmic in unligated form but translocates to the nucleus upon ligand-binding. Can also translocate to the nucleus in unligated form in the presence of RACK1. |
Tissue Location | [Isoform 2]: Mainly expressed in heart and skeletal muscle. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Androgens exhibit a wide range of effects on the development, maintenance and regulation of male phenotype and reproductive physiology in males. The androgen receptor (AR) is a member of the steroid superfamily of ligand-dependent transcription factors. ARs bind active testosterone (T) and dihydrotestosterone (DHT). The rates of association and dissociation of T are about 3 times more rapid than those of DHT. This difference in binding kinetics may account for the different physiological effects of T and DHT. Androgen binding results in an at least 6-fold increase in androgen receptor stability.
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