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Maltose Binding Protein - Tag (MBP-Tag) Antibody (Clone 17D07)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | P59214 |
| Reactivity | All Species |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 κ |
| Clone Names | Clone 17D07 |
| Calculated MW | 45367 Da |
| Positive Control | Western blot: MBP-Tagged protein |
|---|---|
| Application & Usage | ELISA, WB |
| Other Names | MBP-Tag |
| Target/Specificity | MBP-Tag |
| Antibody Form | Liquid |
| Appearance | Colorless liquid |
| Formulation | 1 mg/ml in 0.15 M PBS |
| Handling | The antibody solution should be gently mixed before use. |
| Reconstitution & Storage | -20 °C |
| Background Descriptions | |
| Precautions | Maltose Binding Protein - Tag (MBP-Tag) Antibody (Clone 17D07) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | malX |
|---|---|
| Function | Part of an ABC transporter complex involved in the uptake of maltodextrins. Binds glycogen-derived linear maltooligosaccharides increasing in size from maltotriose to maltooctaose with the highest affinity for maltotriose. Has a very weak affinity for maltose. Has also a very low affinity for maltotetraitol, indicating that the binding is selective for maltooligosaccharides with an intact reducing end. |
| Cellular Location | Cell membrane; Lipid-anchor |
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Provided below are standard protocols that you may find useful for product applications.
Background
Maltose Binding Protein (MBP) tags have demonstrated utility to increase expression and proper folding of proteins expressed in bacteria leading to improved solubility of the partner protein. MBP allows one to use a simple capture affinity step on amylose-agarose columns, resulting in a protein that is often 70-90% pure. In addition to protein-isolation applications, MBP provides a high degree of translation and facilitates the proper folding and solubility of the target protein. MBP fusion proteins can also be expressed in yeast.
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