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ApoF Antibody (Center)

Rabbit Polyclonal Antibody

     
  • WB - ApoF Antibody (Center) ABV11272-100
    APOF Antibody (Center) western blot analysis in CEM cell line lysates (35 μg/lane).This demonstrates the APOF antibody detected the APOF protein (arrow).
    detail
  • IHC - ApoF Antibody (Center) ABV11272-100
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with APOF antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession Q13790
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 35399 Da
Additional Information
Gene ID 319
Positive Control Western blot: CEM cell line, IHC: human hepatocarcinoma tissue
Application & Usage Western blot: ~1:1000, IHC: ~1:10-1:50.
Other Names APOF; Apolipoprotein F; Lipid transfer inhibitor protein
Target/Specificity ApoF
Antibody Form Liquid
Appearance Colorless liquid
Formulation 100 µl of antibody in PBS with 0.09% (W/V) sodium azide
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsApoF Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name APOF
Function Minor apolipoprotein that associates with LDL. Inhibits cholesteryl ester transfer protein (CETP) activity and appears to be an important regulator of cholesterol transport. Also associates to a lesser degree with VLDL, Apo-AI and Apo-AII.
Cellular Location Secreted
Tissue Location Expressed by the liver and secreted in plasma.
Research Areas
Citations (0)
citation

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Background

Apolipoproteins are a family of fatty-acid binding proteins that transport fat through the bloodstream in the form of lipoproteins. ApoF (apolipoprotein F), also known as LTIP (lipid transfer inhibitor protein), is a 308 amino acid secreted protein that belongs to the apolipoprotein family. Expressed in liver tissues and also existing in plasma, ApoF inhibits the activity of CETP (cholesteryl ester transfer protein) and, via this inhibition, functions to regulate cholesterol transport. The gene encoding ApoF maps to human chromosome 12, which encodes over 1,100 genes and comprises approximately 4.5% of the human genome. Chromosome 12 is associated with a variety of diseases and afflictions, including hypochondrogenesis, achondrogenesis, Kniest dysplasia, Noonan syndrome and Trisomy 12p, which causes facial developmental defects and seizure disorders.

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Discontinued
Cat# ABV11272-100
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