|Application ||WB, IHC, IF|
|Calculated MW||35853 Da|
|Positive Control||Western blot: A375 cell line lysates, IHC: human colon carcinoma, IF: 293 cells.|
|Application & Usage||Western blot: ~1:1000, IHC: ~1:10 – 1:50, IF: ~1:10 – 1:50.|
|Other Names||AKR1B1; ALDR1; Aldose reductase; Aldehyde reductase; Aldo-keto reductase family 1 member B1.|
|Formulation||100 µl of antibody in PBS with 0.09% (W/V) sodium azide|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||AKR1B1 Antibody (CT) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.|
|Tissue Location||Highly expressed in embryonic epithelial cells (EUE) in response to osmotic stress.|
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Provided below are standard protocols that you may find useful for product applications.
Aldose reductase (also designated AKR1B1, ALDR1, ALR2 or AR) is member of the monomeric NADPH-dependent aldoketoreductase family. Aldose reductase, which has a molecular mass of 36 kDa, catalyzes the reduction of various aldehydes and has been implicated in the development of diabetic complications by catalyzing the reduction of the aldehyde form of glucose, to the corresponding sugar alcohol, sorbitol. This pathway plays a minor role in glucose metabolism in most tissues, however in diabetic hyperglycemia, cells undergoing insulin-independent uptake of glucose accumulate significant quantities of sorbitol. The resulting hyperosmotic stress to cells may be a cause of diabetic complications such as neuropathy, retinopathy, and cataracts. Aldose reductase is very similar to human aldehyde reductase, bovine prostaglandin F synthase and to the European common frog protein, rho-crystallin.
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