|Application ||WB, IHC, IF|
|Reactivity||Human, Mouse, Rat, Rabbit|
|Calculated MW||39420 Da|
|Positive Control||Western blot: 293, RD, mouse NIH/3T3, rat L6 cell line lysates, IHC: human lung carcinoma, IF: HepG2 cells.|
|Application & Usage||Western blot: ~1:1000, IHC: ~1:10-1:50, IF: ~1:10–1:50.|
|Other Names||ALDOA; ALDA; Fructose-bisphosphate aldolase A; Lung cancer antigen NY-LU-1; Muscle-type aldolase.|
|Formulation||100 µl of antibody in PBS with 0.09% (W/V) sodium azide|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Aldolase A (ALDOA) Antibody (NT) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein (By similarity).|
|Cellular Location||Cytoplasm, myofibril, sarcomere, I band. Cytoplasm, myofibril, sarcomere, M line. Note=In skeletal muscle, accumulates around the M line and within the I band, colocalizing with FBP2 on both sides of the Z line in the absence of Ca(2+)|
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Provided below are standard protocols that you may find useful for product applications.
Fructose bisphosphate aldolase A, also known as Aldolase A is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. It is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Deficiency has been associated with myopathy and hemolytic anemia. Recombinant human Aldolase A, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
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