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PCAF Antibody (NT)

Rabbit Polyclonal Antibody

     
  • WB - PCAF Antibody (NT) ABV11312-100
    Western blot analysis of MDAMB231 line lysate. (35 Jg protein).
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q92831
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 93013 Da
Additional Information
Gene ID 8850
Positive Control Western blot: MDA-MB231 cell lysate
Application & Usage Western blot: ~1:1000.
Other Names KAT2B; PCAF; Histone acetyltransferase KAT2B; Histone acetyltransferase PCAF; Lysine acetyltransferase 2B; P300/CBP-associated factor.
Target/Specificity PCAF
Antibody Form Liquid
Appearance Colorless liquid
Formulation 100 µl of antibody in PBS with 0.09% (W/V) sodium azide
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsPCAF Antibody (NT) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KAT2B {ECO:0000303|PubMed:27796307, ECO:0000312|HGNC:HGNC:8638}
Function Functions as a histone acetyltransferase (HAT) to promote transcriptional activation (PubMed:8945521). Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles (PubMed:8945521). Also acetylates non- histone proteins, such as ACLY, MAPRE1/EB1, PLK4, RRP9/U3-55K and TBX5 (PubMed:9707565, PubMed:10675335, PubMed:23001180, PubMed:27796307, PubMed:23932781, PubMed:26867678, PubMed:29174768). Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A (PubMed:8684459). Acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators: NPAS2-BMAL1 and CLOCK-BMAL1 heterodimers (PubMed:14645221). Involved in heart and limb development by mediating acetylation of TBX5, acetylation regulating nucleocytoplasmic shuttling of TBX5 (PubMed:29174768). Acts as a negative regulator of centrosome amplification by mediating acetylation of PLK4 (PubMed:27796307). Acetylates RRP9/U3-55K, a core subunit of the U3 snoRNP complex, impairing pre-rRNA processing (PubMed:26867678). Acetylates MAPRE1/EB1, promoting dynamic kinetochore-microtubule interactions in early mitosis (PubMed:23001180). Also acetylates spermidine (PubMed:27389534).
Cellular Location Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm Note=Mainly localizes to the nucleus. Also localizes to centrosomes in late G1 and around the G1/S transition, coinciding with the onset of centriole formation. Subcellular location may vary depending upon cell differentiation state. Cytoplasmic at the very stages of keratinocyte differentiation, becomes nuclear at later differentiation stages Cytoplasmic in basal epithelial cells (undifferentiated cells) and nuclear in parabasal cells (differentiated cells) (PubMed:20940255) Localizes to sites of DNA damage (PubMed:25593309)
Tissue Location Ubiquitously expressed but most abundant in heart and skeletal muscle. Also expressed in the skin, in keratinocytes (at protein level) (PubMed:20940255).
Research Areas
Citations (0)
citation

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Background

The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as “readers” of histone acetylation marks regulating the transcription of target promoters. p300/CBP-associated factor (PCAF) is a transcriptional coactivator that works both as a histone lysine acetyltransferase, through its HAT domain, and as an acetyl-lysine reader through its conserved bromodomain located directly C-terminal to the HAT domain. The PCAF bromodomain binds acetylated histone H3 and H4 as well as non-histone targets. Bromodomain binding is dictated by the position of the acetylated lysine as well as interactions with specific residues flanking the acetyl-lysine. PCAF also specifically binds the HIV viral protein Tat on acetylated K50 to regulate its transactivating activity and help induce chromatin remodeling of proviral genes, thereby promoting transcription of viral proteins.

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Discontinued
Cat# ABV11312-100
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